Elispot is now widely used as a method to determine cytokine secretion, especially in vaccine development. The antigen specific secretion of IFN-gamma also is used for diagnostic procedures. We include some references without any claim to completeness. Use medline http://www.ncbi.nlm.nih.gov/entrez/query.fcgi and type "elispot and HIV", " elispot and HCV" etc. to get recent publications online.
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