ELISPOT KITS AND ANTIBODIES

The Enzyme-Linked ImmunoSpot (ELISpot) assay is a very sensitive immunoassay, allowing detection of secreted cytokines at the single cell level. For the detection of human cytokines, chemokines and other immunological effector molecules, Mabtech provides a broad range of EliSpot kits in different formats.

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B-CELL ELISPOT AND ANTIBODIES

In spite of the first described use of the ELISpot being that of detecting antibody-secreting B cells, there have so far not been any designated ELISpot kits for this application. To accommodate this need for validated reagents and protocols we have recently developed a series of kits for detection of human, monkey and mouse Ig-secreting cells.

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MONOCLONAL ANTIBODIES FOR ELISPOT AND ELISA

All monoclonal antibodies used in our highly specific EliSpot and ELISA assay are also available separately in two different sizes.

ENZYME CONJUGATES

SUPPLEMENTARIES FOR ELISPOT

Our highly purified streptavidin-enzyme conjugates are especially suited for enzyme immunoassays.

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FLUOROSPOT

Mabtech has developed a high quality FluoroSpot assay with capacity for simultaneous detection of two different cytokines at the single cell level using detection reagents labeled with green and red fluorochrome.

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Click on species of choice to select your product

B-CELL FLUOROSPOT

The B-cell FluoroSpot is as sensitive as ELISpot but offers the possibility to, in the same well, separately define and enumerate B cells secreting antibodies of different isotypes. This can be done for antigen-specific B-cells or with the purpose to determine the total number of B cells producing different immunoglobulin isotypes.

Mabtech has developed a high quality B-cell FluoroSpot assay for simultaneous detection of two different immunoglobulin isotypes at the single cell level. Detection reagents labeled with green and red fluorophores can be used to determine the number of antigen-specific B cells as well as the total number of Ig-secreting cells.

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Latest Research

The EliSpot Method and Assay Procedure

We have now launched a website dedicated to the ELISpot Assay. Feel free to browse the site and hopefully you will find something interesting. Click here.

EliSpot Applications

The cytokine ELISpot assay has been widely applied to investigate specific immune responses in various diseases including infections, cancer, allergies and autoimmune diseases. It has also become a standard tool in the development and monitoring of new vaccines and vaccine candidates. Often Interferon-gamma (IFN-γ) has been the analyte of choice serving as an immunocorrelate for CD8+ cytotoxic T-cell (CTL) responses