Our Range of EliSpot Readers

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(An example of double stained cells expressing IFN-g and IL-2).

 

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Latest Products

Applications of EliSpot in Diagnosis and Research

Elispot is now widely used as a method to determine cytokine secretion, especially in vaccine development. The antigen specific secretion of IFN-gamma also is used for diagnostic procedures. We include some references without any claim to completeness. Use medline http://www.ncbi.nlm.nih.gov/entrez/query.fcgi and type "elispot and HIV", " elispot and HCV" etc. to get recent publications online.

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EliSpot Classic Reader

iSpot Fluorescence Reader

iSpot Reader Spectrum

vSpot Reader Spectrum

Fast, efficient and user-friendly, the most successful EliSpot reader development in the EliSpot market.

Extremly fast firewire connected digital camera Custom made optics QuadPack 2-axis precision ballbearing stage, impact-protected LED Ring Illumination Speed: ~2 min from insertion of plate to printed end-result Controlled by a high-end PC-system Comes with TFT screen, DVD burner, printer and external storage media 250 mA @ 230 V - 500 mA @ 110 V

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One of the most successful enzymatic EliSpot and FluoroSpot Reader developments in recent years. For the first time, the AID iSpot system allows analysis of both enzymatic and fluorescent (FluoroSpot) based EliSpot assays.

EliSpot and FluoroSpot interpretation LED Ring Illumination, 3&1 filter wheel narrow band fluorescent filters (FITC and Cy3), a third filter one on demand Digital Firewire Camera, 1600x1200 pix, Color Optimized for 2- and 3-colour analysis Software-driven camera-focus Speed:<6 min for an two-color FluoroSpot plate, < 2 min for an EliSpot plate Controlled by a high-end PC-system, incl. TFT screen, DVD burner, printer and external storage media 350 mA @ 230V - 700 mA @ 110V

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The iSpot Spectrum FluoroSpot Reader is the newest generation of the the successful AID iSpot, the first commercially available combined EliSpot/ FluoroSpot Reader. The iSpot Spectrum is equipped with an 7&1 position filter wheel, which allows for a customized selection of up to 7 individual narrow band fluorescent filters, whilst still allowing for our "two click switch," to perform enzymatic analysis via LED illumination. The insertion of a high resolution 5.2 MP digital camera provides well images of unprecedented quality. As with the normal iSpot Reader AID's unique FluoroAID™ image overlay technology permits exact detection of cells secreting multiple cytokines.

Enzymatic and fluorescent EliSpot analysis Digital Firewire Camera, 5.2 MP, Color LED Ring Illumination, 7&1 filter wheel 3 narrow band fluorescent filters (FITC and Cy3), a third one of users choice Optimized for 2- and 3-colour analysis. Up to 7 individual fluorescent filters Automated plate input/output module Controlled by a high-end PC-system, incl. TFT screen, DVD burner, printer and external storage media 350 mA @ 230V - 700 mA @ 110V

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The vSpot Spectrum is the new high end Fluorospot device from AID. It combines AID iSpot 96-well Fluorospot analysing with enzymatic multiple plate analysing. On the enzymatic side the vSpot Spectrum can cope with a variety of different assay types including Viral Plaque Assays and Neutralisation assays. Due to a genuine optical zoom, versatile stage settings and unique software features this reader is not restricted to the analysis of 96 well formats, instead it can handle 6-, 12-, 24-, 48- well plate layouts as well. The insertion of high resolution digital cameras provides well images of unprecedented quality. The vSpot Spectrum is equipped with an 8 position filter wheel, which allows for a customized selection of up to 8 individual narrow band fluorescent filters, whilst still allowing for our "two click switch," to perform enzymatic analysis via LED illumination on different plate formats.

6, 12, 24, 48 and 96 well format Digital Firewire Camera, 5.2 MP, Color LED Ring Illumination, 7&1 filter wheel 3 narrow band fluorescent filters (FITC and Cy3), a third one of users choice Optimized for 2- and 3-colour analysis. Up to 7 individual fluorescent filters Automated plate input/output module Controlled by a high-end PC-system, incl. TFT screen, DVD burner, printer and external storage media 350 mA @ 230V - 700 mA @ 110V

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MultiSpot Reader Spectrum

Robotic EliSpot/FluoroSpot Reader

  

 

The AID multiSpot Reader system fulfills probably all needs in a modern immunology lab. Flexible reader solutions for cell viability tests, cell counting, HLA screening, HEp-2 screening, EliSpot and FluoroSpot appication.

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AID have designed this robotic system for high throughput and traceability of results. This is the ultimate tool for clinical studies involving large groups of samples. AID will also customize the system for your needs. Choose standard, high resolution or iSpot form.

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Fluorescent EliSpot

Why Fluorescent EliSpot?

The EliSpot assay is the most sensitive cellular immunoassays available, allowing the detection of cytokine producing T-cells even at the single cell level.
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Why AID iSpot?

iSpot - a unique EliSpot device capable of analysing Fluorospot and enzymatic-based EliSpot assays.
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AID EliSpot software solutions

Based on the experience and feedback of more than 500 AID EliSpot users all over the globe, we have improved the successful AID EliSpot software and present Version 6.0 of this user-friendly software.
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